Beetroot pigment coursework

Fullscreen The investigation into the effect of temperature on cell membrane permeability in beetroot cells. This pigment is contained in the cell vacuole membrane bound organelle containing water and enzymes.

Beetroot pigment coursework

Yoojin Lee Candidate Nu mber: March 14, Internal Assessment — Determining the relationship between ethanol concentration and rate of diffusion of Betanin pigment of beetroot, using the visible spectrophotometer Research Question: How will changing ethanol concentration affect the rate of diffusion of beetroot pigment, Betanin 1from beetroot cubes placed in water, measured using visible spectrophotometer?

Visible spectrophotometer 2 is a device that measures the absorbance of solutions.

Beetroot pigment coursework

Some wavelengths of light pass through, but some wavelengths of light reflect back. For example, beetroot pigment, which is red in color, reflects wavelengths that code for red and absorbs other wavelengths that code for different colors.

From 0ºC to 20ºC, there’s a slight increase in the average percentage of transmission. This is because when water freezes, it expands and this makes the cell membrane bursts. The bursting results in more pigments leaking out and the darker the colour of the liquid obtained. For example, beetroot pigment, which is red in color, reflects wavelengths that code for red and absorbs other wavelengths that code for different colors. The detector records the reflection of light. The pigment from any cells that have been cut by the cork borer will leak into the water. Rinse away any pigmented water in the morning and replace with fresh water. If you do not have a cork borer, cut the beetroot with a bread slicer (or onion slicer) to make even .

The detector records the reflection of light. Betanin is responsible for the red pigment in a beetroot. It is a glycoside composed of sugar and colored portion.

It is water soluble, which lets diffusion possible in aqueous environment. Betanin is found in vacuoles in plant cells. When the plasma membrane of the plant cell is denatured by ethanol, the Betanin pigments will flow out of the cell, down the concentration gradient.

Beetroot pigment coursework

The beetroot piece after washed with distilled water has its plasma membranes around the cells to protect and to resist leaking of red pigments. However, when ethanol is added to the solution surrounding the beetroot piece, ethanol molecules will destroy the plasma membranes and make red pigments to come out to the solution.

In this experiment, the relationship between ethanol concentration and the rate of reaction, which is represented by absorbance of beetroot pigment, will be tested. In this experiment, the molar absorbtivity and cuvette are constant, because beetroot solution is the only solution to be tested and the same cuvette is used for each trial.

Thus, with two constant variables, the Beer-Lambert Law states that the absorbance is directly proportional to the concentration. However, the linear relationship between the absorbance and the concentration is deviated at high concentrations, so in this experiment, only solutions of low concentration are valid.

Rate of diffusion is represented by the change in absorbance in an hour. Since ethanol has an ability to destroy plasma membrane of beetroot cells, the increase in ethanol concentration will destroy the membranes more severely, which will result in excess diffusion of beetroot pigments from the cell.

The relationship between ethanol concentration and absorbance is directly proportional. Hence, as the ethanol concentration goes up, the rate of diffusion will increase accordingly.From 0ºC to 20ºC, there’s a slight increase in the average percentage of transmission.

This is because when water freezes, it expands and this makes the cell membrane bursts.

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The bursting results in more pigments leaking out and the darker the colour of the liquid obtained. When beetroot is sliced or cut, the cells are also sliced, causing the pigment to spill out.

The thinner the slices are, the larger the surface area, which would increase the rate of pigment leakage. But if the membrane is destroyed and the phospholipid bilayer and are changed, more pigment, betacyanin leaks by means of diffusion.

The beetroot is probably the most popular one this is probably because beetroot contains the red pigment and they are soluble in water, and it’s the betanin in the beetroot that’s responsible for the red pigment.

When beetroot is sliced or cut, the cells are also sliced, causing the pigment to spill out. The thinner the slices are, the larger the surface area, which would increase the rate of pigment leakage.

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Normally the pigments cannot pass through the membrane however when the beetroot is cooked, the colour tends to leak. The aim of this experiment is to use beetroot to examine the effect of temperature on cell membranes and link the observation to the structure of membranes.

Testing the effect temperature has on Beetroot Introduction: Beetroot contains a red pigment name betalains, located in the cell vacuole. Normally the pigments cannot pass through the membrane however when the beetroot is cooked, the colour tends to leak.

Investigating the effect of temperature on plant cell membranes | Nuffield Foundation